Composite

Part:BBa_K2983073:Design

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-15)


hrGFP expression cassette under the control of pTef1 (BBa_K2983052) promoter and Lip2 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

DNA synthesis and assembly

References